The clinical problem.
Pyrexia of Unknown Origin (PUO) remains a clinical challenge despite a greater understanding of the
diseases responsible and increased access to diagnostic tests about. 51% of cases remain undiagnosed,
indicating that there is a need for a methodical, stepwise approach to investigate PUO.
Represents approximate 50% of consultations.
One of the most common reasons for admission to hospital.
Responsible for high mortality and loss of life expectancy.
Conventional algorithm VS ID-Fever Multiplexdiagnosis
Early diagnosis is essential for quick outcomes and management strategies, along with surveillance,
outbreak control and research related to vaccine and drug development.
Patient with suspected infection.
Conventional diagnostic approach.
Symptomatic diagnosis.
Empirical diagnosis.
Science and silk terms consistent with high temperatures a. gives a presumptive idea about the
cause of fever.
Culture.
Significant only in bacterial infections.
Text 72 hours for final result.
Isolation of fastidious organisms has low sensitivity. 25 to 30%.
Transportation half sample decides the viability of bacteria.
Serology.
Takes at least five to seven days for a positive result.
No relevance in active patient management.
MMultiplex PCR: Solution for unmet needs.
ID-Fever Multiplex panels assays simultaneously detect viruses, parasites and bacteria in one
sample, thereby providing a comprehensive patient profile enabling faster and more
appropriate treatment decisions when they mater the most.
**Multiplex PCR differentiates cause of February illness, including malaria radiology
confirmed pneumonia and typhoid fever.**
Differentiation of all four stereotypes of dengue.
Multiplex PCR allows specific detection of dengue virus with unique amplicon. Tm of each
stereotype.
Differentiation of all four species of Plasmodium.
Multiplex PCR accurately detects and identifies plasmodium species in a single reaction with
distinct peaks.